Dengue Virus Detection Kit (Real-time RT-PCR)

[Background]

According to the world health organization, dengue fever is one of the fastest emerging infections and is currently the most rapidly spreading viral disease know.

[Clinical significance]

  • The assay detects DENV serotypes 1,2,3 or 4 from human serum or plasma collected from human patients with signs and symptoms consistent with dengue infection.
  • Any person presenting with symptoms within a week consistent with acute dengue infection or dengue fever including fever, headaches, joint and muscle pain, fatigue nausea, vomiting, rash, and Hemorrhagic manifestations.
  • DENV RNA is detectable in serum from 1-7 days post symptom onset. A positive PCR test is confirmatory of DENV infection. A negative PCR test does not exclude infection as viremia decreases over time and must be followed up with serological testing.

[Advantage]

  • High sensitivity; DENV RNA is detectable in serum once the symptom onset;
  • Open system: Suitable for most real-time PCR thermo cyclers and nucleic acid extraction.

[Recommended thermal cyclers]
-Applied Biosystems TM7500 Real-Time PCR System
-BioRad CFX96 Real-Time PCR System
-Ultrassay XP96/Mini16 Real-Time qPCR System
-Hongshi SLAN-96P Real-Time PCR System
-Other real-time thermal cyclers which could detect FAM/ROX- /VIC(HEX)/CY5 simultaneously

[Types of Sample]
human serum or plasma

[Product specifications] 
RT PCR Kit: 25 tests / kit, 50 tests / kit

Spin Column method:24 tests / kit 50 tests / kit 100 tests / kit

Magnetic bead extraction method: 16 tests / kit 48 tests / kit 96 tests / kit

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