[Research Use Only]
The HCV Quantitative Real-Time PCR Kit is used for detecting and quantitate Hepatitis C Virus (HCV) RNA in human blood plasma or serum samples with an aid of Quantitative Real-Time Polymerase Chain Reaction (qPCR) method.
The hepatitis C virus (HCV) is a small, enveloped, single-stranded, positive-sense RNA virus. It is a member of the genus Hepatitis C virus in the family Flaviviridae. HCV is spread primarily by direct contact with human blood. It is a major cause of acute hepatitis and chronic liver disease, including cirrhosis and liver cancer. Detecting HCV RNA in blood will be helpful in determining the infection of HCV virus and monitoring disease progression and response to antiviral therapy.
This test kit is a quantitative real-time RT-polymerase chain reaction assay for detecting hepatitis C virus (HCV) RNA in human plasma and serum. Nucleic acids of HCV were extracted from plasma/serum using spin column. The conserved region of hepatitis C virus was selected as the detection target gene. The specific primers and fluorescent probe were designed. The fluorophore is attached at the 5′ end of the probe and the quencher moiety is located at the 3′ end. A separate probe with VIC fluorophore at the 5’ end is set as internal control in the assay to rule out the failure of amplification in cases where the target sequence is not detected. The extracted RNA was reversely transcribed into cDNA, and the fluorescence signal was detected by fluorescence quantitative PCR The quantitative detection of RNA in samples can be realized by the change of fluorescent signal value of RNA standard amplification; the processing process of samples to be tested and the existence of PCR inhibition reaction can be monitored by detecting whether the amplification of internal control standard is normal, so as to avoid false negative detection.
- Magnetic bead method for nucleic acid extraction: Automated, high-throughput operation, simple operation,
- High sensitivity: The sensitivity can reach 30IU/mL, and the virus with a load of 10IU/mL can still be detected
- Covering all types: This kit could detect HBV B, C, D genotypes
- High speciﬁcity: No cross-reactivity with other viruses (such as HAV, HCV, HIV, HDV, HEV) which have the same site of infection or similar symptoms of infection.
—ABI7500 Real-Time PCR System
—BioRad CFX96 Real-Time PCR System
—Ultrassay Archimed X4/X6 Real-Time qPCR System
—Other Real-Time PCR System which could detect FAM, VIC/(HEX) simultaneously.
Specimen types: Serum, Plasma.
Packing Speciﬁcations丨50 Tests/Kit