[REF] UBP-H00550H
[Research Use Only]
The HCV Genotyping Real-Time PCR Kit is used for detect and genotype Hepatitis C Virus (HCV) subtypes 1b, 2a, 3a, 3b and 6a in human blood plasma or serum samples. The test results are for laboratory reference only, and the final conclusion should be comprehensively considered in close combination with other results.
Hepatitis C virus (HCV) belongs to the flaviviridae family, Its genome is a single-stranded positive-stranded RNA, which is easily mutated. The virus exists in the hepatocytes, serum leukocytes and plasma of infected persons. The HCV gene is susceptible to mutation and can be divided into at least 6 genotypes and multiple subtypes. HCV genotypes 1b and 2a are relatively common in our country, among which 1b is the main type, followed by 2 and 3. No reports of genotypes 4 and 5 have been reported, and 6 is relatively rare.
[Test Principle]
This detection kit adopts the PCR method combined with the amplification detection technology of fluorescent probes, and selects the NS5B region of hepatitis C virus as the target sequence for detection [2~4]. Select the conserved sequences of different gene subtypes to design the primer probes for detection, and use different fluorescent groups for labeling [5]. Using the fluorescence quantitative PCR detection technology, the change of the fluorescent signal during the PCR process is used to realize the detection of hepatitis C in the sample. Detection of different genotypes of the virus. The detection system contains an internal standard, and the processing of the sample to be tested and whether there is a PCR inhibition reaction in the detection are monitored by whether the nucleic acid amplification of the internal standard is normal, so as to avoid false negatives in the detection.