Human Leukocyte Antigen HLA-B27 Detection Kit (Real-time PCR)

[REF] UBP-G00150H

[Research Use Only]

This kit is suitable for qualitative detection of the DNA in the human leukocyte antigen subtypes HLA-B*2702, HLA- B*2704 and HLA-B*2705.
This kit can be used as an aid for ankylosing spondylitis detection. Ankylosing spondylitis (AS) is a chronic progressive inflammatory disease that mainly affects the spine and can involve the sacroiliac joints and surrounding joints to varying degrees. It has been revealed that AS exhibits obvious familial aggregation and is closely related to human leukocyte antigen HLA-B27. In humans, more than 70 HLA-B27 subtypes have been discovered and identified, and of them, HLA-B*2702, HLA-B*2704 and HLA-B*2705 are the most common subtypes related to the disease [1-10]. In China, Singapore, Japan and Taiwan district, HLA-B27 is most common and accounts for approximately 54%, followed by HLA-B*2705 which accounts for 41% [1-4]. This kit can detect the DNA in HLA-B*2702, HLA-B*2704 and HLA-B*2705, but does not differentiate them from each other.
Experimental operators should have received professional training in gene amplification or molecular biologic testing, and have qualifications relevant to experimental operation. The laboratory should be equipped with rational biological safety precautions and protective procedures.

[Test Principle]
This kit uses fluorescence-based PCR for qualitative detection of the DNA in the subtypes HLA-B*2702, HLA-B*2704 and HLA-B*2705. The HLA-B27 gene is located on the short arm of human chromosome 6. So far, more than 70 HLA-B27 subtypes have been discovered and identified, and there are differences in gene sequence among these subtypes. This kit contains primers and probes specific for HLA-B*2702, HLA-B*2704 and HLA-B*2705 in exon 3; its internal control is designed in the conserved region of the Globin gene and serves as a non-competitive internal standard that can monitor the entire experimental process. This kit contains an enzyme system composed of dUTP/UNG, which can effectively prevent environmental contamination. In the kit, the target gene detection probe is labeled with the fluorophore FAM, and the internal control gene detection probe is labeled with the fluorophore VIC.

[Advantage]

  • Efficient: Simultaneous detection of HLA-B*2702, HLA-B*2704 and HA-B large 27-5 subtype DNA.
  • Specificity: The negative coincidence rate of 10 negative reference products in 10/10.
  • Sensitivity: Limit of detection is 0.3ng/ul.
  • Reliability: Whole process quality control of internal, negative and positive control.

[Applicable Equipment]

—ABI7500 Real-Time PCR System

—Stratagene MX3000P/3005P Real-Time PCR System

—BioRad CFX96 Real-Time PCR System

—Ultrassay Archimed X4/X6 Real-Time qPCR System

—Hongshi SLAN-96P Real-Time PCR System

[Instruction for use]

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