Human Papillomavirus (HPV) Detection Kit (Real-time PCR)

[REF] UBP-C00350H

[Research Use Only]

Ultrassay HPV Detection Kit (Real-time) PCR is used for qualitative detection of 28 types of human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in female cervical exfoliated cells, but is not used for completely genotyping.
Cervical cancer is one of the most common malignant tumors of female reproductive tract. Previous studies have shown that persistent infection and multiple infection of human papillomavirus is one of the important causes of cervical cancer. At present, there is still a lack of recognized effective treetment means for HPV, so early detection and early prevention of cervical HPV are the keys to block cancer. To establish a simple, specific and rapid etiological detection method is of great significance in cervical cancer.
This test cannot be used independently or prior to cervical cytology. It is not recommended to use this test alone for cervical cancer screening in people of any age. This method is not a substitute for cervical cytology and should not be used alone as a basis for patient management. It should not be used for purposes related to cervical cancer screening because no corresponding trials have been conducted.
[TEST PRINCIPLE]
The kit uses multiple nucleic acid amplification (PCR) fluorescence detection method. Highly specific primers and probes are designed based on the L1 gene target sequence of HPV. The specific probe is labeled with FAM fluorophore (HPV6, 11, 16, 18, 31, 54, 56, 83), VIC fluorophore (HPV26, 44, 61, 81 and internal reference), CY5 fluorophore (HPV40, 42, 43, 45, 51, 52, 53, 82) or ROX fluorophore (HPV33, 39, 35, 58, 59, 66, 68, 73) at 5′, and the 3′ quencher group is BHQ1 or BHQ2. During the PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probes bound to the target sequence, it exerts the function of 5′ end exonuclease to separate the reporter fluorophore from the quencher fluorophore, so that the fluorescence monitoring system can receive the fluorescent signal, that is, every time a DNA strand is amplified, a fluorescent molecule is formed, which realizes the complete synchronization of the accumulation of fluorescent signals and the formation of PCR products, so as to achieve qualitative detection of 28 types of human papillomavirus (HPV 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in cervical exfoliated cell samples.

[Advantage]

  • Professional: Nucleic Acid Test is the “gold standard” for Virus Detection
  • Specificity: No cross-reaction with other fusion genes.
  • Sensitivity: Limit of detection to 100 copies/reaction.
  • Reliable: Internal reference and blank control, positive control of the whole process quality control.

[ACCEPTABLE SPECIMENS]

The samples for testing are women’s cervical shedding cells, collected by using cervical shedding cell collectors.

[APPLICABLE EQUIPMENT]

Applicable to Ultrassay XP96/Mini 16 Real time qPCR system, ABI 7500 Real-Time PCR thermocycles, Bio-Rad CFX 96 and other PCR thermocycles which can detect FAM, VIC/HEX, ROX and CY5 fluorescence.

[IFU]

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