Human KRAS Gene Mutation Detection Kit (Real-time PCR)

——Auxiliary diagnostic indicators (DNA) for the individualized treatment plan for cancer patients

[Background]

The K-ras gene is located on chromosome 12 and plays an important role in the growth of human tumors. The normal K-ras gene can regulate the cell growth path. Once the K-ras gene is mutated and it permanently activated, then intracellular signal conduction is disturbed, which leads to continuous cell growth and prevents cell apopto- sis, thereby causing cancer..

K-ras Gene Mutation Frequency:

  • ≈40% Colorectal Cancer
  • ≈90% Pancreatic Cancer
  • ≈15 % Lung Cancer

[Advantage]

  • Easy Operation: Completing the test within 3 hours from submitting the specimen to getting the result;
  • High Accuracy: Reliable results and reducing false negative test results through internal control gene supervision;
  • High Safety: Reducing pollutions through the closed-tube detection ;
  • High Sensitivity: Accurate detection of mutant DNA with concentration as low as 1%, better than direct sequencing method.
  • Strong Specificity: Amplification refractory mutation system combined with Taqman fluorescence quantitative PCR technology, inhibiting wild-type template amplification and improving the specificity of detection

[Clinical Significance]

Once K-ras gene mutations are detected in benign tumor Patient, which indicates the possibility of malig- nant transformation and help doctor to screen effective targeted drugs (such as Erbitux, Panitumumab, etc.) to achieve individualized treatment of tumor patients.

[Applicable Equipment]

—ABI7500 Real-Time PCR System
—Stratagene MX3000P/3005P Real-Time PCR System —BioRad CFX96 Real-Time PCR System
—Ultrassay XP96 Real-Time qPCR System —Hongshi SLAN-96P Real-Time PCR System
—Other Real-Time PCR System which could detect FAM, VIC/(HEX) simultaneously.

Packing Specifications丨12/24 Tests/Kit
Testing Samples 丨 Tumor patient’s lesion tissues(pancreatic cancer, lung cancer, colorectal cancer), including fresh diseased tissue, frozen pathological section, paraffin-embedded tissue or section sample.

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