Leukemia Gene JAK2 V617 Mutation Detection Kit (ARMS-PCR)[Intended use]
The JAK family is a class of non-receptor tyrosine protein kinases, including 4 types of JAK, JAK1, JAK2, JAK3 and TYK2. Some growth factors and most cytokines can activate signal transducers and activators of transcription (STAT) through JAK, thereby affecting gene transcription regulation. JAK-STAT signal transduction pathway is involved in many important biological processes such as cell proliferation, differentiation, apoptosis and immune regulation. Some mutations in JAK2 gene continue to activate the JAK-STAT pathway, which is the cause of tumorigenesis.
Myeloproliferative diseases (MPD) mainly include polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (IMF). It has been confirmed that about 90% of PV and 50% of ET and IMF patients have JAK2 gene V617F point mutation, JAK2 gene mutation detection can be used for the diagnosis of MPD. At present, the World Health Organization (WHO) has included it in the diagnostic criteria of MPD.
[Principles]
The kit adopts ARMS-qPCR method to detect JAK2 V617 codon hot spot mutations.
Design primers and specific probes based on the basic principles of fluorescent PCR to detect the target in a targeted manner, and determine the number (concentration) difference between the analytes in the original sample by analyzing the results after amplification.
[Sample type]
Punctured fresh tissue, Wax block tissue, High-salt soaked tissue, Formaldehyde soaked or fixed tissue, Peripheral blood nucleated cells, Bone marrow puncture.
Plasma is NOT applicable.
[Applicable Equipment]
—ABI7500 Real-Time PCR System
—Stratagene MX3000P/3005P Real-Time PCR System
—BioRad CFX96 Real-Time PCR System
—Ultrassay XP96 Real-Time qPCR System
—Hongshi SLAN-96P Real-Time PCR System