Leukocyte Antigen HLA-B27 Detection Kit

[REF] UBP-G00150H

[Specification] 50 tests/kit

[Intended Use]

UltraDx Leukocyte Antigen HLA-B27 Detection Kit is suitable for qualitative detection of the DNA in the human leukocyte antigen subtypes HLA-B*2702, HLA-B*2704 and HLA-B*2705.

UltraDx Leukocyte Antigen HLA-B27 Detection Kit can be used as an aid for the clinical diagnosis of ankylosing spondylitis. Ankylosing spondylitis (AS) is a chronic progressive inflammatory disease that mainly affects the spine and can involve the sacroiliac joints and surrounding joints to varying degrees.

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[Summary]

It has been revealed that AS exhibits obvious familial aggregation and is closely related to human leukocyte antigen HLA-B27. In humans, more than 70 HLA-B27 subtypes have been discovered and identified, and of them, HLA-B*2702, HLA-B*2704 and HLA-B*2705 are the most common subtypes related to the disease [1-10]. In China, Singapore, Japan and Taiwan district, HLA-B27 is most common and accounts for approximately 54%, followed by HLA-B*2705 which accounts for 41% [1-4]. This kit can detect the DNA in HLA-B*2702, HLA-B*2704 and HLA-B*2705, but does not differentiate them from each other.

The test results obtained by this kit are for clinical reference only, and should not be used as the sole basis for diagnosis or exclusion. Experimental operators should have received professional training in gene amplification or molecular biologic testing, and have qualifications relevant to experimental operation. The laboratory should be equipped with rational biological safety precautions and protective procedures.

[Test Principles]

UltraDx Leukocyte Antigen HLA-B27 Detection Kit uses fluorescence-based PCR for qualitative detection of the DNA in the subtypes HLA-B*2702, HLA-B*2704 and HLA-B*2705. The HLA-B27 gene is located on the short arm of human chromosome 6. So far, more than 70 HLA-B27 subtypes have been discovered and identified, and there are differences in gene sequence among these subtypes. This kit contains primers and probes specific for HLA-B*2702, HLA-B*2704 and HLA-B*2705 in exon 3; its internal control is designed in the conserved region of the Globin gene and serves as a non-competitive internal standard that can monitor the entire experimental process. This kit contains an enzyme system composed of dUTP/UNG, which can effectively prevent environmental contamination. In the kit, the target gene detection probe is labeled with the fluorophore FAM, and the internal control gene detection probe is labeled with the fluorophore VIC.

[Advantages]
  • Rapid detection: DNA extraction 20 mins + Amplification 60 mins.
  • Strong Specificity: Non-cross reactivity with other subtypes of HLA gene.
  • Easy operation: Compare to flow cytometry, PCR test not required viable lymphoid.
  • Contamination control: dUTP/UNG systems to avoid carry over contamination of PCR products in subsequent amplification reactions.
  • High sensitivity: 1ng/uL genomic DNA
  • Internal control system: Prevent false negative results.
[Acceptable Specimens]

Applicable sample type: EDTA anti-coagulated whole blood.

[Applicable Equipment]

Applied Biosystems™ Real time PCR system 7500, ABI QuantStudio™5 Real-time PCR system, LightCycler® 480 PCR system, Bio-Rad CFX96 real-time PCR instrument. Ultrassay eQ9600 Real Time qPCR System etc.