The Ultrassay PIK3CA Gene Mutation Detection Kit is a sensitive and selective assay for the detection of five mutations in the phosphoinositide-3-kinase catalytic subunit alpha (PIK3CA) gene.
It has been found that PIK3CA gene mutations exist in several types of cancers, including breast cancer and non-small-cell lung cancer. Mutation of PIK3CA may lead to persistent activation of the downstream signaling pathway associated with the development and spread of cancer.
The PIK3CA five mutation kit is a real-time PCR assay. It employs mutation-specific primers and a PIK3CA-targeted proprietary fluorescent probe to detect low-copy number (1%) PIK3CA-mutant DNA in cancer tissue.
The kit enables detection of the following mutations against a background of wild-type genomic DNA.
• E542K (1624G>A)
• E545D (1635G>T)
• E545K (1633G>A)
—ABI7500 Real-Time PCR System
—Stratagene MX3000P/3005P Real-Time PCR System
—BioRad CFX96 Real-Time PCR System
—Ultrassay XP96 Real-Time qPCR System
—Hongshi SLAN-96P Real-Time PCR System