Human PIK3CA Gene Mutation Detection Kit (Real-time PCR)

[Intended use]

The Ultrassay PIK3CA Gene Mutation Detection Kit is a sensitive and selective assay for the detection of five mutations in the phosphoinositide-3-kinase catalytic subunit alpha (PIK3CA) gene.

It has been found that PIK3CA gene mutations exist in several types of cancers, including breast cancer and non-small-cell lung cancer. Mutation of PIK3CA may lead to persistent activation of the downstream signaling pathway associated with the development and spread of cancer.

[Principles]

The PIK3CA five mutation kit is a real-time PCR assay. It employs mutation-specific primers and a PIK3CA-targeted proprietary fluorescent probe to detect low-copy number (1%) PIK3CA-mutant DNA in cancer tissue.

The kit enables detection of the following mutations against a background of wild-type genomic DNA.

• H1047R(3140A>G)

• H1047L(3140A>T)

• E542K (1624G>A)

• E545D (1635G>T)

• E545K (1633G>A)

[Applicable Equipment]

—ABI7500 Real-Time PCR System

—Stratagene MX3000P/3005P Real-Time PCR System

—BioRad CFX96 Real-Time PCR System

—Ultrassay XP96 Real-Time qPCR System

—Hongshi SLAN-96P Real-Time PCR System

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