Sars-CoV-2, Influenza, Respiratory Syncytial Virus Detection Kit (Real-time PCR)
Cat. No.: UP-RSFR-50
Ultrassay Sars-CoV-2, Influenza, Respiratory Syncytial Virus detection kit is intended for qualitative detection of ORF1ab gene of 2019-Novel Coronavirus (2019-nCoV, SARS-COV-2), M1 gene or NS1 gene of Influenza Virus and F gene of RSV in the specimens including Oropharyngeal swabs, sputum and Bronchoalveolar lavage fluid.
The novel coronaviruses belong to the β genus. COVID-19 is an acute respiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source of infection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases.
Influenza is a kind of highly acute respiratory infectious diseases caused by the infection of influenza virus, it can be suddenly onset and rapidly spread in a short term, resulting in different degrees of prevalence, with high incidence rate and a certain mortality rate. The symptoms caused by influenza A and B virus are similar. The most obvious characteristics are acute high fever and cough. Other symptoms include chills, headache, muscle ache, fatigue, nasal congestion and runny nose, etc. IFA has the strongest antigenic variation, which often causes worldwide pandemic. IFB has a relative weak variability, generally causes mild diseases, mainly attacks children, and may cause local outbreaks.
Human respiratory syncytial virus (RSV) is a single-stranded, negative-strand, RNA virus belonging to the Paramyxoviridae family, and is in the genus Pneumovirus. RSV is one of the most common viruses to infect children worldwide and increasingly is recognized as an important pathogen in adults, especially the elderly. The most common clinical scenario encountered in RSV infection is an upper respiratory infection, but RSV commonly presents in young children as bronchiolitis, a lower respiratory tract illness with small airway obstruction, and can rarely progress to pneumonia, respiratory failure, apnea, and death.
The test is manually operated. Experimental personnel who perform this test should have received professional training in gene amplification or molecular biology diagnostics and be qualified for relevant experimental operations. There should be reasonable biosecurity precautions and protective procedures in the laboratories. The test should only be performed in laboratories that follow safety practices according to the applicable Biosafety Regulations in Microbiological and Biomedical Laboratories.
The results of this kit are only for aiding to diagnosis and shall not be used as the sole basis for diagnosis or exclusion.
This kit uses polymerase chain reaction (PCR) technology based on TaqMan fluorescent probe to detect ORF1ab gene of 2019-Novel Coronavirus, M1 gene or NS1 gene of Influenza Virus and F gene of RSV from specimens including Oropharyngeal swabs, sputum and Bronchoalveolar lavage fluid collected from the patients or suspected patients.
Nasopharyngeal swab, oropharyngeal swabs, bronchoalveolar lavage fluid, saliva, sputum or fecal
Applicable to Ultrassay XP96/Mini 16 Real time qPCR system, ABI 7500 Real-Time PCR thermocycles, Bio-Rad CFX 96.