PCR, Polymerase Chain Reaction, is a method for enzymatic synthesis of specific DNA fragments in vitro. It consists of high temperature denaturation, low temperature annealing (renaturation) and suitable temperature extension. The cycle is carried out, and the target DNA can be rapidly amplified. It has the characteristics of high specificity, high sensitivity, simple operation and time saving. Thermal cycler is the device for rapid amplification of genes. Based on Peltier technology, it realizes the rapid, accurate and automatic circulation of reagent temperature, thus realizing the full automation of the PCR process. It can be used not only in basic research such as gene isolation, cloning and nucleic acid sequencing, but also in clinical diagnosis of diseases.
The experienced soft design inhibits the nonspecific amplification of reagents. In practice, if the PCR reagent is put into the block immediately before the hot lid temperature reaches the set value, the reagent in the block will also heating-up with hot lid. Here, the reagent in block will begin to react slowly. But this is not the desired, that is, nonspecific amplification. What to do? during the hot lid warming up, the block is cooled to 10℃ until the hot lid temperature get set value before running the cycle program.
Actually, not all PCR experiments require the fastest ramp rate. Some PCR reactions require fixed heating rate or cooling rate to ensure the result of PCR reaction. After long-term technical accumulation and algorithm optimization, ETC811 provides a nearly perfect linear temperature control curve.
In order to speed up the temperature balance between block and reagent and shorten the reaction time of PCR, the control mode of overheating block temperature is often used in the temperature control algorithm of PCR. However, for small-volume reaction of PCR, excessive overheating will make reagent temperature close to boiling point, which will cause reagent evaporation or even drying, leading to the abandonment of previous work. With the accumulation of temperature control technology for many years, the reagent of ETC811 PCR will not evaporate even in a small volume of 5-15ul, so as to ensure the success of the experiment.
When gradient function is used to optimize the PCR reaction conditions, there are two variables, time and temperature. When the temperature gradient is set, we tend to focus only on the effect of temperature on the PCR reaction. In fact, the time of arrival of temperature is different for each column well. This raises the question: is the result of optimization a function of temperature or time? ETC811 uses a special temperature control algorithm to solve this problem well. By eliminating the time variable, the gradient PCR optimization experiment is only related to temperature, which greatly improves the certainty of the test results.
● Press and lock simultaneously
● Damped cover shaft can be stopped whenever you want
● Adaptable to different tubes (except full skirt)
In engineering mode, a special interface (need password) is designed to show all sensor states for machine debugging and maintenance to accurately judge faults and shorten maintenance time.
|2||Block||0.2ml*96 Aluminum||384 Aluminum||In-situ or flat|
|3||Applicable Consumables||96 Microplate (non-skirt/semi-skirt, except full skirt)Single/8-strip/12-strip tubes (tall/short/flat/dome tubes)||384 Microplate||Customizable|
|4||Input Power||100-240V～50/60Hz, 600VA|
|5||Dimension (L*W*H)||Product size: 427*288*227 mm Carton size: 540*450*385mm|
|6||Weight||Net Weight: 9.7kg Gross weight: 14.5kg|
|7||Temperature control accuracy||≦ ±0.1°C|
|8||Block temperature precision||≦ ±0.1°C|
|9||Block temperature uniformity||≦ ±0.3°C @ 55°C|
|10||Max. ramp rate||≥ 5°C/s|
|11||Avg. ramp rate||≥ 2°C/s|
|12||Temperature Control Technology||3 Peltier independently control. Distributed auxiliary heating on block edge.|
|13||Block control mode||Block/Tube|
|14||Block temperature range||4-99°C|
|15||Hot lid temperature range||30-110°C, ON/OFF|
|16||Real-time curve||Display block and hot lid temperature curve|
|17||Gradient range||30-99°C / 0.1-42°C|
|18||Temperature touch down||0.1-9.9°C/cycle|
|19||Time touch down||1-9m59s/cycle|
|20||Smart ramp rate control technology||Gradient temperature reaches the target simultaneously.|
|21||Ramp rate control||Yes, 0.1-4°C/s adjustable|
|22||Inhibit non-specific amplification||Yes|
|23||Display screen||7″ WVGA 64000 color touch screen with led backlight|
|24||Max PCR step||30|
|25||Memory||Max 1296 files|
|27||Memory function of Power-off||Yes|
|28||Self-adaptable lid||Hot lid can automatically adapt to various heights of tubes (tall /short/flat/dome tubes).|
|29||Self-test||Yes. Self-test every time when power on.|
|30||Auto report||Yes. Auto report every action in process after running.|
|31||Fault self-diagnosis and report||Yes. Fault self-diagnosis and display error code in pop-up window|
|32||Auto soaking after running||Yes. Reagents are auto cooled and keep to 8°Cafter running|
|33||Estimation of remaining time||Yes|
|34||Edit other programs in operation||Yes|
|35||Service support||Yes. Provide technical support and service online|