NRAS Mutation Detection Kit

[REF] UBP-T01012K

[Packing specification] 12Tests/Kit

[Intended use]

UltraDx NRAS Mutation Detection Kit can be used to qualitatively detect mutations of codon 12, 13 and 61 of NRAS gene in tissue samples of patients with malignant tumors (lung cancer, colorectal cancer, pancreatic cancer, nasopharyngeal carcinoma and other solid tumors), so as to provide the basis for individual drug therapy. NRAS gene mutation is associated with primary drug resistance of targeted drugs to EGFR signal transduction pathway in patients with malignant tumors. Patients with wild type of NRAS gene are likely to benefit from the treatment of Erbitux, (also known as Cetuximab) or Vectibix (also known as Panitumumab), Iressaor Tarceva, while malignant tumor patients with NRAS gene mutation do not significantly benefit from such targeted drug therapy.

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[Summary]

This kit can be used to qualitatively detect mutations of codon 12, 13 and 61 of NRAS gene in tissue samples of patients with malignant tumors (lung cancer, colorectal cancer, pancreatic cancer, nasopharyngeal carcinoma and other solid tumors), so as to provide the basis for individual drug therapy. NRAS gene mutation is associated with primary drug resistance of targeted drugs to EGFR signal transduction pathway in patients with malignant tumors. Patients with wild type of NRAS gene are likely to benefit from the treatment of Erbitux, (also known as Cetuximab) or Vectibix (also known as Panitumumab), Iressaor Tarceva, while malignant tumor patients with NRAS gene mutation do not significantly benefit from such targeted drug therapy.

[Test Principles]

UltraDx NRAS Mutation Detection Kit uses the ARMS-PCR method to detect the gene mutation, and uses the relative CT value to determine the genotype. The labeled fluorescent groups are FAM, VIC and Texas Red fluorescence signals.

[Advantages]
  • High sensitivity: Accurate detection of NRAS mutation as low as 5% and 50ng/reaction.
  • Strong Specificity: The specificity can be reached 99.5% by using PCR technology.
  • Easy operation: results in 90 mins.
  • Reliable: Internal and External controls are included.
[Acceptable Specimens]

Applicable sample type: Patient’s diseased tissue samples, including formaldehyde soaked or fixed tissue, high-salt soaked tissue, punctured fresh tissue, FFPE.

[Applicable Equipment]

Applied Biosystems™ Real time PCR system 7500, ABI QuantStudio™5 Real-time PCR system, LightCycler® 480 PCR system, Bio-Rad CFX96 real-time PCR instrument. Ultrassay eQ9600 Real Time qPCR System etc.