——Auxiliary diagnostic indicators (DNA) for the individualized treatment plan for cancer patients

[Purpose]

Ultrassay B-raf V600E Gene Mutation RT PCR test kit is used for the detection of mutations in tumor-specific oncogene gene B-raf V600E, and can be used for individualized molecular diagnosis of tumors, thereby guiding individualized clinical treatment plans for tumor patients.

Mutation Frequency of B-raf Gene V600E:

• 60%～80% Malignant Melanoma
• 29%～83% Thyroid Cancer
• 6%～15% Colorectal Cancer
• 1 % ～3 % Lung Cancer

[Advantage]

• High Sensitivity: Accurate detection of B-raf gene V600E site mutation as
  low as 1%;
• Strong Specificity: Dual blocking technology by using ARMS primers and blocking probes, effectively increasing the specificity of detection;
• Easy Operation: Completing the test within 3 hours from submiting the
  specimen to geting the result;
• High Accuracy: Reliable results and reducing false negative test results through internal control gene supervision.

[Clinical Significance]

• Medication Guide 1: Patients with wild-type K-ras gene and mutations in B-raf gene show ineffective treatment with EGFR inhibitors such as cetuximab and panitumumab
• Medication Guide 2: Panitumumab and Erbitux have significant curative effect in patients with wild-type tumors of B-raf gene
• Prognostic Indicators:
  B-raf mutation is not only a predictive indicator, but also a prognostic indicator. The colorectal cancer patients with B-raf mutation has a poor prognosis.

[Applicable Equipment]

—ABI7500 Real-Time PCR System
—Stratagene MX3000P/3005P Real-Time PCR System—BioRad CFX96 Real-Time PCR System
—Ultrassay XP96 Real-Time qPCR System —Hongshi SLAN-96P Real-Time PCR System

Packing Specifications丨12/24 Tests/Kit
Testing Samples 丨 Tumor patient’s lesion tissue (papillary thyroid cancer, malignant melanoma, colorectal cancer, lung cancer), including fresh diseased tissue, frozen pathological section, paraffin-embedded pathological tissue or section.