Bacteria Genomic DNA Extraction Kit (Spin Column)
[Specification] 50 preps/kit
Ultrassay Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.
- Fast: the whole process can be completed in 50 minutes
- High yield: DNA yield up to 20 μg
- [Storage Conditions and Shelf Life]RNase A at -20°C for 24 months, others at room temperature (15-25°C) for 12 months.
Gram-positive or Gram-negative bacteria cells ≤109.