Feyond Series Multi-Mode Microplate Reader - Ultrassay Life Science Instrument

Feyond Series Multi-Mode Microplate Reader | A300/A400/A500 - Ultrassay
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Feyond Series Multi-Mode Microplate Reader – A300/A400/A500 Models

Ultrassay Feyond Series Multi-Mode Microplate Reader is a versatile, high-performance detection instrument available in three models: A300, A400, and A500. Each model offers customizable detection modes including absorbance (ABS), fluorescence intensity (FI), and luminescence (LUM), designed to meet diverse life science research and clinical laboratory requirements. With its modular design and flexible configuration options, the Feyond series delivers exceptional value and performance.

Three Models to Choose From

Feyond A300 – Basic Absorbance Mode

  • Detection Mode: Absorbance (ABS) only
  • Wavelength Range: 200-1000 nm (filter-based)
  • Ideal For: ELISA, protein quantification, enzyme kinetics, cell viability (MTT, CCK-8)
  • Best For: Basic laboratory applications, routine absorbance measurements

Feyond A400 – Absorbance + Fluorescence

  • Detection Modes: Absorbance (ABS) + Fluorescence Intensity (FI)
  • Fluorescence Excitation: 320-850 nm (selectable filters)
  • Fluorescence Emission: 400-850 nm (selectable filters)
  • Sensitivity: ≤10 fmol fluorescein
  • Ideal For: DNA/RNA quantification, calcium flux assays, TRF, HTRF, GPCR assays
  • Best For: Advanced research requiring both absorbance and fluorescence detection

Feyond A500 – Full Multimode (ABS + FI + LUM)

  • Detection Modes: Absorbance (ABS) + Fluorescence Intensity (FI) + Luminescence (LUM)
  • Luminescence Range: 300-700 nm
  • Sensitivity: Attomole levels of luciferase
  • Ideal For: Reporter gene assays, ATP cell viability, BRET assays, glow-type luminescence
  • Best For: Comprehensive research requiring all three detection modes

Key Features (All Models)

  • Modular Design: Choose the model that fits your application needs and budget
  • High-Sensitivity PMT Detector: (A400/A500) Detects low-light signals with exceptional signal-to-noise ratio
  • Flexible Reading Modes: Top read, bottom read, or dual read (A400/A500)
  • Filter-Based Optical System: Dedicated filters for specific excitation/emission wavelengths
  • Flexible Plate Compatibility: Supports 96-well and 384-well microplates
  • Temperature Control: Incubation up to 45°C for cell-based assays
  • Orbital Shaking: Ensures thorough mixing before measurements
  • 10.4-inch Color Touch Screen: Intuitive graphical interface for standalone operation
  • Professional Software: ReaderIt-II with quantitative analysis, standard curve fitting, and data export
  • Dual-Language Interface: Available in Chinese and English

Applications by Model

A300 Applications

  • ELISA: Infectious disease detection, cytokine measurement
  • Protein Quantification: Bradford, BCA, Lowry assays
  • Cell Viability: MTT, CCK-8, cell proliferation assays
  • Enzyme Kinetics: Time-course measurements

A400 Applications

  • All A300 applications
  • DNA/RNA Quantification: Nucleic acid analysis (A260/A280)
  • Fluorescence Assays: Calcium flux, GPCR activation
  • TRF/HTRF: Time-resolved fluorescence, homogeneous time-resolved fluorescence
  • Biomarker Research: Disease biomarker detection

A500 Applications

  • All A400 applications
  • Reporter Gene Assays: Luciferase, GFP expression
  • ATP Cell Viability: Bioluminescence-based cell counting
  • BRET Assays: Bioluminescence resonance energy transfer
  • Drug Discovery: High-throughput screening (HTS)

Why Choose Ultrassay Feyond Series?

  • ✓ Flexible Configuration: Choose from 3 models to match your needs and budget
  • ✓ Upgradeable: A300 can be upgraded to A400 or A500 as your research grows
  • ✓ Cost-Effective: Pay only for the detection modes you need
  • ✓ High Performance: PMT detector and xenon flash lamp ensure accurate results
  • ✓ Easy to Use: Touch screen and professional software for flexible operation
  • ✓ Reliable: Xenon flash lamp with long service life (>10 years)
  • ✓ Professional Support: Free lifetime technical support via email, phone, WhatsApp
  • ✓ 1-Year Warranty: Manufacturer warranty with optional extension
  • ✓ Fast Delivery: Standard products shipped within 3-5 business days

Which Model Should You Choose?

  • Choose A300 if: You primarily need absorbance measurements for ELISA and routine assays
  • Choose A400 if: You need both absorbance and fluorescence for advanced research applications
  • Choose A500 if: You require all three detection modes for comprehensive research or drug discovery

Request a Quote

Interested in the Feyond Series Multi-Mode Microplate Reader? Contact us for a personalized quote and technical consultation to help you choose the right model.

📧 Email: info@ultrassay.com | 📱 WhatsApp: +86 186 5516 6990

UV / Vis Absorbance

Wavelength selection is done by using an advanced monochromator system. Any wavelength between 200 to 1000 nm can be selected. Using the spectral scanning feature, the whole spectrum of a sample be scanned in 1 nm increments to allow identification of the optimal measurement wavelength for a new assay.

  • Long life xenon lamp which can be used for 10*9
  • Fast reading mode only need time 15 s for 96-well whole plate
  • Can be used for spectral scanning, endpoint and kinetic detection
UV,Vis-1
Fluorescence

Feyond Series is equipped with filter-based fluorescence optics and dichroic mirrors for screening applications such as fluorescence polarization, and TR-FRET. Standard applications such as fluorescence-based DNA/RNA quantification assays are not only supported in microplates, but also in low volume u-Nano ultra-micro plate.
The independent removable filter modules make it more convenient for users to replace the filter. The filter-based fluorescence optics detection ensures high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent blockage of undesired wavelengths. This is ideal for excitation and emission applications. The filters are also the technically preferred and most cost efficient technology for non-absorbance based assays.

Fluoresence-1
Luminescence

Feyond series luminescence microplate readers show excellent sensitivity and wide dynamic range in both glow and flash based assays. The PMT enhances the maximum sensitivity of weak luminescence signals, prevents over-saturation of high signals, effectively improves the detection range of luminescence. The optimized light path minimizes signal crosstalk between holes and ensures the accuracy of experimental results. The precise dual-channel injector ensures assay performance even when assaying high-density 384-well plates.

Luminescence-1
Time-Resolved Fluorescence (TRF)

Time-resolved fluorescence is based on lanthanide elements as dyes. When excited, the emission time is much longer than that of ordinary fluorescein. After the excitation light is turned off, the emitted light can still be continuously expressed and released, thereby eliminating the interference of excitation light and scattered light.
Time-resolved fluorescence has high sensitivity, strong specificity, good stability, and short operation flow. It is suitable for ultra-micro analysis in biology and medicine, hormone detection, viral hepatitis marker detection, target cell marker detection, and drug screening.

TRF-1
Fluorescence Polarization (FP)

The optimized optical path design of Feyond-A500 combined with the perfor-mance of the fast switching polarizer can effectively reduce the detection deviation. The 10-inch lower computer touch screen can improve the flexibility of experimental parameter setting. This function is often used to detect the interaction between small molecules and macromolecules, such as the determination of drugs and hormones, tyrosine kinase detection, receptor/ligand research, protein/polypeptide interactions, DNA/protein interactions, etc.

FP-1
User Authority Classification

User permissions are divided into four levels, with clear permissions to avoid the occurrence of misuse by researchers;

Administrator can manage the accounts of different sub-users;

The users have independent accounts and passwords to ensure the safety and confidentiality of experimental results.

Feyond multimode reader touch screen - 10.4 inch display for standalone operation with multiple detection modes
Intuitive Interface Display

Intuitive selection of function modes, easy parameter setting;

The layout colors are distinguished clearly, convenient to be checked for the position of the sample;

Programs and results are stored independently, making it easy to be found the required applications.

Feyond multimode reader touch screen - 10.4 inch display for standalone operation with multiple detection modes
Powerful Data Analysis and Process

Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis to help you obtain the analysis results you want;

Algorithm customization: according to your assays needs, you can customize the required algorithm, tailor-made for convenient assays.

Powerful Data Analysis and Process-1
Liberalized User Communication

The software has with a shared library, which can store the program, results and standard curves for sharing them with others;

The program and standard curve can be created in real time to a QR code, and the required content can be imported only by scanning the code with the instrument.

Feyond A300 A400 A500 comparison - Three models of multimode microplate readers with different detection modes
FTP (File Transfer Protocol)

Upload the data directly to a computer with a FTP server, and users can view the data results at any time in the authorized folder.

FTP (File Transfer Protocol)-1
Technical Specifications
Model Feyond-A300 Feyond-A400 Feyond-A500
Absorbance Light source Xenon lamp
Detector PD
Wavelength accuracy 2 nm
Wavelength repeatability 0.2nm
Half width <2.5 nm
Wavelength range 200-1000 nm, 1 nm step
Measure range 0-4 OD
Resolution 0.0001 OD
Accuracy @450nm 96-precision mode: ± (1.0 %+0.003 Abs) @(0.0-2.0 Abs]

±2.0 % @(2.0-3.0 Abs]
Repeatability @450nm CV < 1.0 % or SD < 0.003 fast (0.0-3.0Abs]

CV < 0.5 % or SD < 0.003 accurate (0.0-3.0Abs]
Stray light 0.1% @220 nm
Linear @450nm R2 ≥ 0.999 @[0.0 – 3.0 Abs]
Reading time 96-well plate: fast <15 s
Luminescence Detector PMT
Detection limit 15 amol/hole; 5 amol/hole (photon PMT)
Linear dynamic range 6 logs
Crosstalk ≤ 0.005 %
Wavelenth range 200-850 nm
Fluorescence Reading mode Top reading
Excitation light

source

 Xenon lamp
Detector PMT
Wavelength range EX: 200-1000 nm; EM: 270-850 nm
Filter EX/EM 3 groups: EX470/EM525, EX523/EM564, EX624/EM692 (other wavelengths can be replaced)
Detection limit 1 pM
Linear dynamic

range

 6 logs
TRF Wavelength range – EX: 200-1000 nm; EM: 270-850 nm
Detection limit – 0.05 pM
FP Wavelength range – – λ1-λ2
Detection limit – – 5 mP
Basic Parameter
Model Feyond-A300 Feyond-A400 Feyond-A500
Plate 6-384 well
Accessories Microplate, injector
Shaking mode Linear, annular, double annular
Incubation temperature RT+4 °C ~ 45 °C
Temperature uniformity ±0.5 °C @37 °C
Software interface Chinese / English
Screen size 10-inch
Operation method Capacitive screen touch, mouse
Data capacity 10 GB
Compatibility Support PC software, Win7/Win10 64 bit
Network transmission The test data report can be uploaded to the PC server through FTP
Instrument port 2 USB Type A ports, 1 USB Type B port, 1 Ethernet port, Rs232 bus interface (connected to the injector)
Power supply AC 100-240 V, 50-60 Hz
Size (W×D×H) 420×550×386 mm
Weight 33 kg

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