Human Immunodeficiency Virus (HIV) Detection Kit (Real-time PCR)
[Research Use Only]
The human immunodeficiency virus nucleic acid assay kit (hereinafter referred to as the Kit) is used for the quantitative detection of human immunodeficiency virus RNA in human serum or plasma samples.
The human immunodeficiency virus (HIV), which lives in a person’s blood, can destroy the body’s immune system, thus making the body lose its ability to fight off other diseases, causing incurable infections and tumors, and eventually death. HIV can be transmitted through sexual contact, blood and from mother to child.
This kit is suitable for the quantitative detection of HUMAN immunodeficiency virus RNA in serum/plasma samples, and can monitor the HIV level in the blood of human immunodeficiency virus patients, providing an auxiliary means for the detection of human immunodeficiency virus patients; The test results are for research use only, and the final conclusion should be considered in conjunction with other indicators.
This kit should not be used for blood source screening.
This kit uses a combination of PCR amplification and fluorescence probe to design specific detection primers and fluorescence probe for the conserved regions of human immunodeficiency virus. The probe was an oligonucleotide labeled with 5 ‘FAM fluorescence group and 3’ BHQ1 quenching group. When the probe was intact, the fluorescence signal emitted by the reporter group was absorbed by the quenched group. During PCR amplification, the 5 ‘-3’ exonuconase activity of Taq enzyme will degrade the probe enzyme and separate the reporting and quenching fluorophore, so that the fluorescence signal can be received by the fluorescence monitoring system. That is, for each AMPLIFIED DNA strand, a fluorescence molecule is formed, and the accumulation of fluorescence signal is fully synchronized with the formation of PCR products. The extracted RNA was reverse transcribed into cDNA, and quantitative fluorescence PCR detection technology was used to detect the RNA in the sample by the change of fluorescence signal in the PCR process and the change of fluorescence signal value of RNA standard amplification. At the same time, exogenous internal reference was added to control the quality of reagent, DNA and operation itself to avoid false negative.
Applicable to Ultrassay Archimed X4/X6/Mini 16 Real time qPCR system, ABI 7500 Real-Time PCR thermocycles, Bio-Rad CFX 96.