Sars-CoV-2 (ORF1ab, E&N Gene Detection Kit (Real-time RT-PCR)

Cat. No.: UP-RS2G3-50


Ultrassay Sars-CoV-2 detection kit is intended for the qualitative detection of ORF1ab, E & N genes of 2019-Novel Coronavirus (2019-nCoV, SARS-COV-2) in the specimens including nasopharyngeal swab, oropharyngeal swabs, bronchoalveolar lavage fluid, saliva, sputum, and fecal. The ORF1ab gene sequences are higher specificity for 2019-nCoV, while the sequences of the E and N genes are homogenous to the same genes in some viruses such as SARS-like coronaviruses and coronaviruses carried by some bats.
The novel coronaviruses belong to the β genus. COVID-19 is an acute respiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source of infection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases.
This kit is only for emergency use during the pandemic of 2019-nCoV. The test is manually operated. Experimental personnel who perform this test should have received professional training in gene amplification or molecular biology diagnostics and be qualified for relevant experimental operations. There should be reasonable biosecurity precautions and protective procedures in the laboratories. The test should only be performed in laboratories that follow safety practices according to the applicable Biosafety Regulations in Microbiological and Biomedical Laboratories.
The results of this kit are only for aiding to diagnosis and shall not be used as the sole basis for diagnosis or exclusion.


This kit uses polymerase chain reaction (PCR) technology based on TaqMan fluorescent probe to detect ORF1ab, E & N genes of 2019-Novel Coronavirus from specimens including oropharyngeal swabs, Nasopharyngeal swab, bronchoalveolar lavage fluid, saliva, fecal and sputum collected from the patients or suspected patients.


Nasopharyngeal swab, oropharyngeal swabs, bronchoalveolar lavage fluid, saliva, sputum or fecal


Applicable to Ultrassay XP96/Mini 16 Real time qPCR system, ABI 7500 Real-Time PCR thermocycles, Bio-Rad CFX 96.